Amahora ayisithupha okuchayeka entuthuni kagwayi kubangele ukufa kwamangqamuzana okuhlola, kuyilapho ukuchayeka okufanayo kumhwamuko we-e-cigarette akuzange kuphazamise ukusebenza kwezicubu.
Ihlolwe kusukela ezinhlotsheni ezimbili ezihlukene zama-e-cigarette, umhwamuko okhiqizwayo wawungenawo umthelela we-cytotoxic ezicutshini zomoya womuntu, ngokusho kocwaningo olusha olushicilelwe ku-In Vitro Toxicology (DOI: 10.1016/j.tiv .2015.05.018).
Ososayensi be Ugwayi waseMelika waseBrithani et Inkampani MatTek Corporation wasebenzisa inhlanganisela eyingqayizivele yokuhlola ukuze atadishe imiphumela emibi engaba khona yomhwamuko we-e-cigarette kuzicubu zepheshana lokuphefumula futhi wawuqhathanisa nentuthu kagwayi. "Ngokusebenzisa umshini wentuthu kanye nokuhlola okusekelwe elabhorethri kusetshenziswa izicubu zokuphefumula, bekungenzeka ukukala umthamo ocasulayo we-aerosol kanye nokuqinisekisa ukuthi ama-aerosol ahlukahlukene akhona ku-e-cigarette asetshenziswe kulolu cwaningo awanawo umphumela. izicubu zepheshana kubantu “Kusho umkhulumeli UDkt Marina Murphy.
Le ndlela entsha ingasetshenziswa ukusiza ukuthuthukisa izindinganiso ezintsha zalezi zinhlobo zemikhiqizo esikhathini esizayo.
Umhwamuko okhiqizwa ugwayi we-elekthronikhi ungase ube ne-nicotine, ama-humectants, ama-flavorings kanye nemikhiqizo yokuwohloka kokushisa, ngakho-ke kubalulekile ukuqonda umthelela ongaba khona ezinhlelweni zebhayoloji. Kuze kube manje, azikho izifundo ezifakazela imiphumela emibi engaba khona ye-e-cigarette vapor kumamodeli we-in vitro alingisa ngokuphelele ukwakheka, ukusebenza kanye nokuchayeka kwezicubu ezijwayelekile zokuphefumula komuntu.
Abacwaningi bahlanganise imodeli ye-3D etholakala ngokwentengiso yezicubu ze-epithelial zokuphefumula kanye nerobhothi "Vitrocell" elivame ukusetshenziselwa lolu hlobo lokuhlola "nomusi" ukuze kuhlolwe amandla okucasuka komhwamuko we-e-cigarette wamamodeli amabili atholakala ngokwentengiso. Imiphumela ibonisa ukuthi, naphezu kwamahora aqhubekayo okuchayeka, umthelela womhwamuko we-e-cigarette ezicutshini zepheshana lokuphefumula ufana nowomoya. Ngaphezu kwalokho, lolu cwaningo lumele umnyakazo wokuqala obheke ekuhlalisaneni kwabantu futhi lwethula inkulumo-mpikiswano mayelana nemihlahlandlela engaba khona embonini.
Imodeli yethishu yepheshana lokuphefumula " I-EpiAirway ihlanganisa amangqamuzana e-tracheal/bronchial epithelial akhuliswe ukuze akhe izingqimba ezihlukene ezifana nezicubu ze-epithelial zepheshana lokuphefumula. Uhlelo " I-Vitrocell ilingisa ukuchayeka ehogweni yomuntu ngokunikeza idatha yokukhipha ugwayi noma ugwayi we-e. Ingase futhi ithumele ukuhogela emuva ezicutshini. I-EpiAirway".
Abacwaningi baqale bahlola isistimu yebhayoloji ngezinto ezicasulayo ezaziwayo ezisetshenziswa oketshezi. Bese beveza izindwangu I-EpiAirway entuthuni kagwayi nama-aerosols akhiqizwa ezinhlotsheni ezimbili ze-e-
ugwayi amahora ayisithupha. Ngalesi sikhathi, ukusebenza kwamaseli kukalwa njalo ngehora kusetshenziswa i-colorimetric assay esunguliwe. Inani lenqwaba yezinhlayiyana elifakwe endaweni yeseli nalo lalinganiswa (kusetshenziswa amathuluzi e-dosimetry) ukuze kuqinisekiswe ukuthi intuthu noma umhwamuko ufinyelele esicutshini ngesikhathi sonke sokuchayeka.
Imiphumela ibonisa ukuthi i- intuthu kagwayi yehlisa ukusebenza kwamaseli ku-12% (eduze nokufa kwamaseli ngokuphelele) ngemva kwamahora ayisithupha. Ngokuphambene, awekho ama-aerosol e-e-cigarette abonise ukwehla okuphawulekayo ekusebenzeni kwamaseli. Ngaphandle kwamahora angu-6 okuchayeka okuqhubekayo, imiphumela ibifana namaseli okulawula avezwe emoyeni kuphela . Futhi ngisho nokuchayeka okunamandla, umhwamuko we-e-cigarette awukunciphisi ukusebenza kwamaseli.
«Okwamanje, awekho amazinga aphathelene nokuhlolwa kwe-in vitro yama-e-cigarette aerosols”, kusho uMarina Trani, inhloko ye-R&D yemikhiqizo ye-nicotine yesizukulwane esilandelayo se-British American Tobacco. Kodwa uyanezela,Iphrothokholi yethu ingaba usizo kakhulu ekusizeni inqubo ukuthi iye phambili.»
Lolu cwaningo lubonisa ukuthi, kule modeli yezicubu zokuphefumula komuntu, i-cytotoxicity ayithinteki i-e-cigarette aerosols, kodwa izifundo ezengeziwe zizodingeka ukuze kuqhathaniswe imiphumela yeminye imikhiqizo ehlukene etholakalayo kwezohwebo, amafomethi kanye nokwakheka.
Umthombo : Eurekalert.org
